A brief documentation to how dbGaP website is accessed & how to download data.

Contributors

Gao Wang, Dongyue Xie

Data directory

Files downloaded from dbGaP need to be decypted & extracted to usable formats. Files are first to be downloaded to some temporary directory, then processed and saved into a more permanent location. It is therefore suggested that on RCC Midway, for example, you download data to a temporary folder under /scratch/midway2/USER then extract them to shared computational space /project2/mstephens/ for sharing with the group. This documentation assumes this workflow. dbGaP access 1. Visit dbGaP, click on Controlled Access Data, then login with your eRA Commons account. You’ll see the dataset previously applied for and approved under the tab Authorized Access -> My Requests. 2. In the column Actions click Request Files for the dataset you want to download. You should see two possibilites in two tabs: • “Phenotype and Genotype files” • “SRA data (reads and reference alignments)” Download “Phenotype and Genotype files” Click on the tab Phenotype and Genotype files, then click / select data file names. After files you need are selected, create the data download request. You should be directed to a download instruction page. You should also receive an email confirmation which contains a link to this page. For example, to download V8 eQTL release, use the Request for Exchange Area data then check the + sign to unfold directory and select data to download, in my case: gtex/exchange/GTEx_phs000424/exchange/analysis_releases/GTEx_Analysis_2017-06-05_v8/eqtl The download instruction page offers several options but I find it most straightforward to use Linux command tool ascp. ascp can be download for Linux then install using bash command. It requires root to install which we don’t have on RCC Midway. But it is easy to hack the installer: just open it with a text editor, eg vim and edit the first 10 lines of code to remove root requirement and change installation path, then save and run bash to install. You should find two files under a bin folder in the path you just edited to the installer: 1) the executable ascp and 2) the license file aspera-license. If you don’t know how to hack it, you can use a version I got on Midway /project2/mstephens/software/ascp (the license file sits in the same directory as ascp). Additionally you need an ssh key which comes from the installation but again I provide it at /project2/mstephens/software/asperaweb_id_dsa.openssh. Having ascp executable alone without these additional files is not going to work. Now you can run the download command. On the instruction page the command might look like: "%ASPERA_CONNECT_DIR%\bin\ascp" -QTr -l 300M -k 1 -i "%ASPERA_CONNECT_DIR%\etc\asperaweb_id_dsa.openssh" -W A7A8C74EB00A14C92826A8EC64785904771847404B340486B977DD32F5F3701B399760E2328AA4F43ADB2A6E66A2EFA22D dbtest@gap-upload.ncbi.nlm.nih.gov:data/instant/gaowang/72550 . but in our case you with ascp set up as previously discussed, you should change it to: /project2/mstephens/software/ascp -QTr -l 300M -k 1 -i /project2/mstephens/software/asperaweb_id_dsa.openssh -W A7A8C74EB00A14C92826A8EC64785904771847404B340486B977DD32F5F3701B399760E2328AA4F43ADB2A6E66A2EFA22D dbtest@gap-upload.ncbi.nlm.nih.gov:data/instant/gaowang/72550 /scratch/midway2/dyxie/dbGaP/ where in this example /scratch/midway2/dyxie/dbGaP/ is your previous setup for download directory. Then you will find your downloaded file under a folder 72550 under that directory, as implied in commands above. Note: we need absolute path to asperaweb_id_dsa.openssh not relative path such as ./ – that won’t work! Ask Kushal he learned the lesson the hard way. The downloaded data have .ncbi_enc file extensions. We need sra-toolkit to decode the data next. Install sra-toolkit If use Anaconda on midway2, then it does not allow user to install sra-toolkit. So we need to install the conda by ourthelves: First download and install Miniconda3 if you haven’t done that before. Replace %Download address% to the download link that you can find here: wget %Download address% Then run bash Miniconda3-latest-Linux-x86_64.sh -bfp /scratch/midway2/USER/miniconda3
echo "export PATH=/scratch/midway2/$USER/miniconda3/bin:\$PATH" >> ~/.bashrc
source ~/.bashrc

Check if using the right conda

which conda

Then the sra tool can be installed via:

conda install -c bioconda sra-tools

Set dbGaP repository key for the project

In order to decrypt, a dbGaP repository key is also required. The key is provided in a file with suffix “.ngc”. It can be obtained from two places in PI’s dbGaP account.

1. The first place is the project page under “My Projects” tab, through a link named “get dbGaP repository key” in the “Actions” column. The key downloaded from here is valid to all downloaded data under the project.

I get the key from the first place. Then config the key by running command

vdb-config --import ~/prj_3253_D17102.ngc

After configuration, manually change the mkfg file:

vim ~/.ncbi/user-settings.mkfg

Then change the root to the data folder you store the data. For example, here, the download directory is /scratch/midway2/dyxie/dbGaP/. See below the change I made to point to that directory.

15c17
< /repository/user/protected/dbGaP-3253/root = "/home/dyxie/ncbi/dbGaP-3253"
---
> /repository/user/protected/dbGaP-3253/root = "/scratch/midway2/dyxie/dbGaP/"

Finally, run

vdb-decrypt --decrypt-sra-files . /path/to/destination -v # we can use somewhere under /project2/mstephens for the otput destination directory

Then all the *.ncbi_enc files under /path/to/downloaded/data will be decrypted and the original *.ncbi_enc files will be automatically removed.